Amanda Tabbert
From Shiu Lab
Academic
- Michigan State University BS Biological Sciences May 2008
- Dortha E. and John D. Withrow Endowed Scholarship in Education 2008
- Robert K. Burnett Memorial Endowed Scholarship 2007
- Michigan Merit Award 2004
- Dean's List: Fall 2005, Spring 2006, Fall 2006, Fall 2007, Spring 2008
I graduated from the College of Education Secondary Science Certification program in May 2009. I am certified to teach Biology (DA) and Integrated Science (DI). Starting in September 2009 I will be teaching biology part-time at Holt High School in Holt, MI.
About Me
I enjoy kayaking, camping, hiking, reading, and traveling overseas.
Protocols
Seed Sterilization: 1)100% bleach for 3 minutes with a drop of tween. 2)Shake 3) centrifuge for 4 seconds 4) pipette off supernatant and discard 5)add 1000 micro liters sterile water and repeat steps 2-5 four times or until soap bubble are gone.
Plating seeds: when plating seeds for germination use 0.1% agar solution instead of water. 0.1 gram agar per 100 ml water.
Basta selection: For a 325 micromolar solution add 6.5 mg of Glufosinate ammonium to 100 mL of water.
1% gel: 200mL of 1xTAE buffer for every 2 grams agarose (1% gel); Add stir bar. Microwave for 1.5 minutes, when cooling add ethidium bromide (4 micro liters per 100 mL of gel). When cool to touch, while stirring, pour gel into mold.
Hyg Plates: follow MS + Sucrose protocol using only 1/2 MS and no vitamins. Add 200 microliter HYG per 500 ml of sol'n when it has cooled to the touch after autoclaving (21 minutes liquid cycle); stir w/ magnetic stir bar, pour plates.
50xTAE: 242g Trizma dissolved in 700ml dH2O then add 57.1 ml glacial acetic acid (in corrosive cabinet under hood)next add 100ml 0.5M EDTA (pH 8.0). Adjust final volume to 1 liter.
